Whole-genome Sequence Analysis to Confirm the Absence of Transgene in a Rice Line Made by Gene Targeting

The recent development of homologous recombination-mediated gene targeting (GT) techniques has made it easy to modify nucleotide sequences in plant genomes. Backcross breeding following GT provides transgene-free lines with high probability. However, owing the possibility unintentional transgene introduction during genetic transformation, analytical methods may be necessary reliably and comprehensively detect transgenes remaining on a genome. Herein, we conducted an analysis based whole-genome sequencing. We analyzed rice DNA using next-generation sequencer, performed data find integration All results supported absence rice. Our genome integrity evaluation method could also applicable produced other genome-editing technologies such as CRISPR/Cas9.